Examinando por Autor "La Manna, Vincenzo"

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A microsatellite study on the genetic distance between suri and huacaya phenotypes in peruvian alpaca (Vicugna pacos)
(2012-01) La Manna, Vincenzo; La Terza, Antonietta; Dharaneedharan, S.; Ghezzi, Silvia; Arumugam Saravanaperumal, S.; Apaza, Nolberto; Huanca Mamani, Teodosio; Bozzi, Riccardo; Renieri, Carlo
Two coat phenotypes exist in alpaca: the Huacaya and the Suri. The two coats have different textile characteristics and different prices on the market. Although present scientific knowledge suggests a simple genetic model of inheritance, there is a tendency to manage and consider the two phenotypes as two different breeds. A 14-microsatellite panel was used in this study to assess genetic distance between Suri and Huacaya alpacas in a sample of non-related animals from two phenotypically pure flocks at the Illpa-Puno experimental station in Quimsachata. Peru. The animals are part of a germplasm established approximately 20 years ago and have been bred separately according to their coat type since then. Genetic variability parameters were also calculated. The codominant data was statistically analysed using the software Genalex 6.3, Phylip 3.69 and Fstat 2.9.3.2. The sample was tested for Hardy-Weinberg equilibrium (HWE) and after strict Bonferroni correction only one locus (LCA37) showed deviation from equilibrium (P<0.05). Linkage disequilibrium (LD) was also tested and 9 loci associations showed significant disequilibrium. Observed heterozygosis (Ho= 0.766; SE=0.044), expected heterozygosis (He=0.769; SE=0.033), number of alleles (Na=9.667, SE=0.772) and Fixation index (F=0.004; SE=0.036) are comparable to data from previous studies. Measures of genetic distance were 0.06 forNei’s and 0.03 for Cavalli-Sforza’s. The analysis of molecular variance reported no existing variance between populations. Considering the origin of the animals, their post domestication evolution and the reproductive practices in place, the results suggest that there is no genetic differentiation between the two populations for the studied loci.
Analysis of genetic distance between Peruvian Alpaca (Vicugna Pacos) showing two distinct fleece phenotypes, Suri and Huacaya, by means of microsatellite markers
(Taylor & Francis, 2016-02-19) La Manna, Vincenzo; La Terza, Antonietta; Ghezzi, Silvia; Saravanaperumal, Siva; Apaza, Nolberto; Huanca Mamani, Teodosio; Bozzi, Riccardo; Renieri, Carlo
Two coat phenotypes exist in Alpaca, Huacaya and Suri. The two coats show different fleece structure, textile characteristics and prices on the market. Although present scientific knowledge suggests a simple genetic model of inheritance, there is a tendency to manage and consider the two phenotypes as two different breeds. A 13 microsatellite panel was used in this study to assess genetic distance between Suri and Huacaya alpacas in a sample of non-related animals from two phenotypically pure flocks at the Illpa-Puno experimental station in Quimsachata, Peru. The animals are part of a germplasm established approximately 20 years ago and have been bred separately according to their coat type since then. Genetic variability parameters were also calculated. The data were statistically analyzed using the software Genalex 6.3, Phylip 3.69 and Fstat 2.9.3.2. The sample was tested for Hardy-Weinberg equilibrium (HWE) and after strict Bonferroni correction only one locus (LCA37) showed deviation from equilibrium (P<0.05). Linkage disequilibrium (LD) was also tested and 9 loci associations showed significant disequilibrium. Observed heterozygosis (Ho= 0.766; SE=0.044), expected heterozygosis (He=0.769; SE=0.033), number of alleles (Na=9.667, SE=0.772) and Fixation index (F=0.004; SE=0.036) are comparable to data from previous studies. Measures of genetic distance were 0.06 for Nei’s and 0.03 for Cavalli-Sforza’s. The analysis of molecular variance reported no existing variance between populations. Considering the origin of the animals, their post domestication evolution and the reproductive practices in place, the results do not show genetic differentiation between the two populations for the studied loci.
Inheritance of white, black and brown coat colours in alpaca (Vicuna pacos L.)
(Elsevier, 2011-05-10) Valbonesi, Alessandro; Apaza, Nolberto; La Manna, Vincenzo; Gonzáles Castillo, Mario Lino; Huanca Mamani, Teodosio; Renieri, Carlo
An experimental trial of the segregation of white vs. pigmented and black vs. brown colours in alpacas was conducted at the Peruvian INIA Quimsachata Experimental Station. One hundred and forty five offspring were born from the following matings: 4 white sires × 36 white dams, 4 white sires × 39 pigmented dams, and 9 pigmented sires × 70 pigmented dams. Among these last matings were, 4 black sires × 25 black dams, 2 black sires × 20 brown dams, and 3 brown sires × 25 brown dams. Statistical tests validate that the inheritance of white is due to a single gene which is dominant over pigmentation, without any modifying effect and independent of segregation of black and brown patterns. However, the evidence does not support a simple dominant inheritance of the black vs. brown.
Suri/Huacaya phenotype inheritance in alpaca (Vicugna pacos)
(2012-01) Renieri, Carlo; Valbonesi, Alessandro; Antonini, M.; La Manna, Vincenzo; Huanca Mamani, Teodosio; Apaza, Nolberto; Presciuttini, Silvano; Asparrin, M.
The Suri/Huacaya phenotype inheritance in alpaca was tested on two independent Peruvian sources of records: the Registry of Mallkini farm (588 offspring by Suri sire x Suri dam from 62 paternal half sib families, and 2,126 offspring by Huacaya sire x Huacaya dam from 177 paternal half sib families) and the results of the Quimsachata INIA ILLPA Puno experimental trial (two reciprocal experimental test-crosses, involving a total of 17 unrelated males and 149 unrelated females). The data support a genetic model in which two linked loci must simultaneously be homozygous for recessive alleles in order to produce the Huacaya phenotype. The estimated recombination rate between these loci was 0.099 (95% C.L. = 0.029-0.204). The birth of 3 Suri offspring from Huacaya x Huacaya mating is explained by a new dominant mutation on some germinal lines of Huacaya animals. The direct mutation rate can be estimated at 0.0014.