Artículos científicos

URI permanente para esta colecciónhttps://repositorio.inia.gob.pe/handle/20.500.12955/8

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Clonación y filogenia molecular de un segmento del gen codante de la actina de Myrciaria dubia "camu camu": un candidato para gen de referencia
(Universidad Científica del Perú, 2012-12-28) Castro Gómez, Juan Carlos; Cobos Ruiz, Marianela; Egoavil Reátegui, Alina del Carmen; Ramírez Saavedra, Roberson; Imán Correa, Sixto Alfredo; Adrianzén Julca, Pedro M.; Marapara del Aguila, Jorge Luis
[ES] Myrciaria dubia “camu-camu” es un frutal amazónico caracterizado por su amplia variación de vitamina C. Pero los estudios genético moleculares que puedan explicar esta variación son limitados. Por ello nuestro objetivo fue realizar la clonación y filogenia molecular de un segmento del gen codante de la actina de M. dubia. Las muestras fueron obtenidas de la colección de germoplasma del INIA. Luego, el ARN fue purificado y mediante RT-PCR con cebadores degenerados se amplificó un segmento del gen. En base a la secuencia obtenida se diseñaron cebadores específicos para PCR en tiempo real. Los resultados muestran que se ha aislado, clonado y secuenciado un segmento del gen codante de actina de M. dubia y detectado su expresión en hojas, pulpa y cáscara de M. dubia. Así, con el soporte de herramientas bioinformáticas y uso de técnicas de biología molecular hemos aislado, clonado y secuenciado un segmento del gen codante de la actina de M. dubia. Asimismo, los análisis realizados muestran que el gen se expresa y presenta niveles similares de expresión en hojas, pulpa y cáscara de M. dubia. Sin embargo, es necesario realizar más experimentos a fin de verificar su estabilidad de expresión. ---- [EN] Myrciaria dubia “camu-camu” is an amazonian fruit tree characterized for its ample variation in vitamin C. But molecular genetic studies that explains this variation are limited. Hence our objective was to accomplish cloning and molecular phylogeny of the segment of actin coding gene from M. dubia. The samples were obtained from germoplasma's collection of the INIA. Next, the ARN was purified and by RT-PCR with degenerate primers was amplified a segment of the gene. After, on the basis of the sequence obtained, specific primers were designed for real time PCR. Results showed that was isolated, cloned and sequenced a segment of actin gene coding from M. dubia. Thus with the support of bioinformatics tools and molecular biology techniques we have isolated, cloned and sequenced a segment of actin coding gene from M. dubia. Additionally, analyses showed that the gene have similar expression levels in leaves, pulp and peel from M. dubia. However, it is necessary to realize more experiments in order to verify its expression stability.
Dataset of de novo assembly and functional annotation of the transcriptome during germination and initial growth of seedlings of Myrciaria Dubia “camu-camu”
(Elsevier, 2020-06-11) Castro Gómez, Juan Carlos; Maddox, J. Dylan; Rodríguez, Hicler N.; Castro, Carlos G.; Imán Correa, Sixto Alfredo; Cobos Ruiz, Marianela; Paredes, Jae D.; Marapara del Aguila, Jorge Luis; Braga, Janeth; Adrianzén Julca, Pedro M.
Myrciaria dubia “camu-camu” is a native shrub of the Amazon that is commonly found in areas that are flooded for three to four months during the annual hydrological cycle. This plant species is exceptional for its capacity to biosynthesize and accumulate important quantities of a variety of health-promoting phytochemicals, especially vitamin C [1], yet few genomic resources are available [2]. Here we provide the dataset of a de novo assembly and functional annotation of the transcriptome from a pool of samples obtained from seeds during the germination process and seedlings during the initial growth (until one month after germination). Total RNA/mRNA was purified from different types of plant materials (i.e., imbibited seeds, germinated seeds, and seedlings of one, two, three, and four weeks old), pooled in equimolar ratio to generate the cDNA library and RNA paired-end sequencing was conducted on an Illumina HiSeq™2500 platform. The transcriptome was de novo assembled using Trinity v2.9.1 and SuperTranscripts v2.9.1. A total of 21,161 transcripts were assembled ranging in size from 500 to 10,001 bp with a N50 value of 1,485 bp. Completeness of the assembly dataset was assessed using the Benchmarking Universal Single-Copy Orthologs (BUSCO) software v2/v3. Finally, the assembled transcripts were functionally annotated using TransDecoder v3.0.1 and the web-based platforms Kyoto Encyclopedia of Genes and Genomes (KEGG) Automatic Annotation Server (KAAS), and FunctionAnnotator.

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Examinando Artículos científicos por Autor "Adrianzén Julca, Pedro M."