Artículos científicos

URI permanente para esta colecciónhttps://repositorio.inia.gob.pe/handle/20.500.12955/8

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A new species of Trechisibus from Peruvian Andes (Coleoptera: Carabidae, Trechinae)
(Sapienza University of Rome, 2016-06-30) Delgado Mamani, Pedro Ernesto; Ruiz Tapiador, Ildefonso
[EN] In this work the new species Trechisibus (s. str.) delestali sp. n., is described from the southern Peruvian Andes. The morphological differences with the geographically closest species of the subgenus are also presented and discussed.
A pathogen complex between the root knot nematode Meloidogyne incognita and Fusarium verticillioides results in extreme mortality of the inka nut (Plukenetia volubilis)
(Julius Kühn-Institut., 2021-10-12) Guerrero Abad, Juan Carlos; Padilla Domínguez, Ammer; Torres Flores, Elías; López Rodríguez, Carlos; Guerrero Abad, Roger Arbildo; Coyne, Danny; Oehl, Fritz; Corazon Guivin, Mike Anderson
The combined infection of the root knot nematode Meloidogyne incognita and two Fusarium species led to high plant mortality of inka nut (Plukenetia volubilis) seedlings in pots after 80 days growth in two independent inoculation experiments. Inoculation of M. incognita juveniles and conidia of F. solani or F. verticillioides, simultaneously, increased plant mortality by 25-30%, compared with M. incognita alone (5-10% mortality). When inoculated with F. verticillioides at 20 days post nematode inoculation, plant mortality increased to 55%. Either of the Fusarium spp. alone caused some plant mortality, but less than when combined with M. incognita. The synergistic interaction of M. incognita and especially F. verticillioides demonstrates a lethal outcome for inka nuts. It appears that M. incognita creates favorable conditions that lead to enhanced effect and damage by the Fusarium species, especially F. verticillioides, leading to devastating levels of plant death.
A review of silvopastoral systems in the Peruvian Amazon region
(2022-05-31) Fuentes, Eduardo; Gómez, Carlos; Pizarro, Dante; Alegre, Julio; Castillo, Miguel; Vela, Jorge; Huaman, Ethel; Vásquez Pérez, Héctor Vladimir
Livestock in the Peruvian Amazon region is mostly produced in areas considered degraded pasturelands and associated with deforestation. Silvopastoral systems (SPS) are an alternative for sustainable livestock production. This article aims to provide information about progress in the development of SPS in the Peruvian Amazon region during the last 2 decades and opportunities to develop it further at the national level. The geographical characteristics and climatic conditions of the Peruvian Amazon are described, followed by a review of the experiences with SPS in the 5 most relevant departments of the region. Constraints for implementation of SPS practices in the country and the current initiatives at regional and national level to promote and develop more sustainable livestock production in the region are presented. There is a large variation in SPS practiced along the different departments of the Amazon region. It is imperative that the Peruvian Government continues promoting SPS for recovering degraded lands through generating enabling conditions for farmers to adopt and/or scale up SPS.
Abono líquido e insecticidas para el control de Spodoptera Frugiperda y rendimiento de maíz
(Universidad Juárez Autónoma de Tabasco, 2022-11-08) Díaz chuquizuta, Percy; Hidalgo Meléndez, Edison; Aguirre Gil, Oniel Jeremías; Valdés Rodríguez, Ofelia Andrea
El objetivo del estudio fue determinar la respuesta de Spodoptera frugiperda y el rendimiento del maíz ante aplicaciones de abono líquido de origen bovino (Biol) con insecticidas biológicos y sintéticos. Se utilizó un diseño de bloques completos al azar con ocho tratamientos (control, Biol, Beauveria bassiana, aceite de Neem, Spinosad, Biol con B. bassiana, Biol con aceite de Neem y Biol con Spinosad). Se utilizó ANOVA y comparaciones de medias de Tukey (p ≤ 0.05) para evaluar resultados. Los menores porcentajes de incidencia de S. frugiperda se obtuvieron con Spinosad ((20.40%) y Biol con Spinosad (12.87%); mientras que el control presentó una incidencia del 65.86% (p < 0.05). Los mayores rendimientos se obtuvieron con Biol, aceite de Neem, Biol con B. bassiana y Biol con aceite de Neem, con 8.15, 7.97, 7.52 y 7.37 t ha−1,respectivamente (p ≥ 0.05);el menor rendimiento se obtuvo al aplicar Biol con Spinosad(p < 0.05).
Acaulospora aspera, a new fungal species in the Glomeromycetes from rhizosphere soils of the inka nut (Plukenetia volubilis L.) in Peru
(Julius Kühn-Institut, 2019-10-09) Corazon Guivin, Mike Anderson; Cerna Mendoza, Agustín; Guerrero Abad, Juan Carlos; Vallejos Tapullima, Adela; Carballar Hernández, Santos; Alves da Silva, Gladstone; Oehl, Fritz
A new fungal species of the Glomeromycetes, Acaulospora aspera, was isolated from the rhizosphere of the inka nut (Plukenetia volubilis) in San Martín State of Peru (Western Amazonia) and propagated in bait cultures on Sorghum spp., Brachiaria brizantha, Medicago sativa and P. volubilis as host plants. The fungus forms brownish yellow to yellow brown spores, (120-)135-195 × (120-)130 187 μm in diameter. The surface of the structural spore wall layer is crowded with small depressions, 0.4-0.7 μm in diameter, up to 0.8 μm deep, and only 1.1-1.8 apart, giving the spore surface a rough, washboardlike appearance, especially when the outermost, evanescent wall layer has disappeared. Phylogenetically, the new species is close to A. spinosissima, A. excavata and to other morphologically more similar species such as A. spinosa and A. tuberculata, which form spiny or tuberculate projections on the outermost, semi-persistent spore wall layer, or A. herrerae, A. kentinensis, A. scrobiculata and A. minuta, which on the structural spore wall layer all have more pronunced pits than A. aspera. In this study, also the name of A. spinosissima was validated, as it had been preliminary declared invalid because of a typing error in the diagnosis section of its original description.
Acaulospora flava, a new arbuscular mycorrhizal fungus from Coffea arabica and Plukenetia volubilis plantations at the sources of the Amazon river in Peru
(Julius Kühn-Institut, 2021-08-06) Corazon Guivin, Mike Anderson; Vallejos Tapullima, Adela; De la Sota Ricaldi, Ana María; Cerna Mendoza, Agustín; Guerrero Abad, Juan Carlos; Santos, Viviane Monique; Da Silva, Glandstone Alves; Oehl, Fritz
A new arbuscular mycorrhizal fungus, Acaulospora flava, was found in coffee (Coffea arabica) and inka nut (Plukenetia volubilis) plantations in the Amazonia region of San Martín State in Peru. The fungus was propagated in bait cultures on Sorghum vulgare, Brachiaria brizantha and Medicago sativa as host plants. It differentiates typical acaulosporoid spores laterally on sporiferous saccule necks. The spores are light yellow, bright yellow to yellow brown, (95-)105-160 × (95-)100-150 μm in diameter and have smooth spore surfaces. Phylogenetically, A. flava clusters in a well-separated clade, nearest to A. kentinensis, followed by A. herrerae, A. spinosissima, A. excavata and A. aspera, of which remarkably A. spinosissima, A. excavata and A. aspera had also been found in inka nut plantations of San Martín State during the last years. Here, we report also A. herrerae and A. fragilissima as fungal symbionts within the rhizosphere of coffee and the inka nut. The later two fungi had so far been recorded by concomitant morphological and molecular analyses only from tropical islands, A. herrerae from Cuba in the Golf of Mexico and A. fragilissima from New Caledonia in the southwest Pacific Ocean close to Australia. In this study, the ITS region of A. herrerae was analyzed for the first time and deposited in the public databases. In total, we already recovered fourteen Acaulospora species from coffee and inka nut plantations in San Martín State of Peru, suggesting that Acaulospora species are frequent and beneficial symbionts in coffee and inka nut roots in San Martín State of Peru.
Acciones para la caracterización y conservación del bovino criollo Peruano (Bos taurus)
(Organización de las Naciones Unidas para la Alimentación y la Agricultura - FAO, 2007-04-01) Rivas Seoane, Emma Rosa; Veli Rivera, Eudosio Amancio; Aquino Villasante, Yeny Natali; Rivas Palma, Victoria Esther; Pastor, Santiago; Estrada Zúniga, Rigoberto
Se describen las actividades de conservación y caracterización del bovino criollo peruano en el Instituto Nacional de Investigación y extensión Agraria (INIEA), las mismas que se han desarrollado con la activa interacción con las comunidades rurales de las regiones peruanas de Ancash, Apurímac, Ayacucho, Junín y Puno, dedicadas a la crianza de ganado criollo; en las comunidades también se llevaron a cabo trabajo de campo y charlas participativas sobre la conservación de sus morfotipos locales. Las actividades desarrolladas incluyen la caracterización morfológica y molecular empleando microsatélites y RFLP para detectar genotipos de proteínas lácteas (variantes de kappa caseínas y beta lactoglobulinas). Esta información será de utilidad para los programas de mejoramiento del bovino criollo peruano en las comunidades rurales.
Adapting agriculture to climate change: A synopsis of coordinated national crop wild relative seed collecting programs across five continents
(MDPI, 2022-07-13) Eastwood, Ruth J.; Tambam, Beri B.; Aboagye, Lawrence M.; Akparov, Zeynal I.; Aladele, Sunday E.; Allen, Richard; Amri, Ahmed; Anglin, Noelle L.; Araya, Rodolfo; Arrieta-Espinoza, Griselda; Asgerov, Aydin; Awang, Khadijah; Awas, Tesfaye; Barata, Ana Maria; Kwasi Boateng, Samuel; Magos Brehm, Joana; Breidy, Joelle; Breman, Elinor; Brenes Angulo, Arturo; Burle, Marília L.; Castañeda-Álvarez, Nora P.; Casimiro, Pedro; Chaves, Néstor F.; Clemente, Adelaide S.; Cockel, Christopher P.; Davey, Alexandra; De la Rosa, Lucía; Debouck, Daniel G.; Dempewolf, Hannes; Dokmak, Hiba; Ellis, David; Faruk, Aisyah; Freitas, Cátia; Galstyan, Sona; García, Rosa M.; Ghimire, Krishna H.; Guarino, Luigi; Harker, Ruth; Hope, Roberta; Humphries, Alan W.; Jamora, Nelissa; Ahmad Jatoi, Shakeel; Khutsishvili, Manana; Kikodze, David; Kyratzis, Angelos C.; León-Lobos, Pedro; Liu, Udayangani; Mainali, Ram P.; Mammadov, Afig T.; Manrique-Carpintero, Norma C.; Manzella, Daniele; Mohd Shukri, Mat Ali; Medeiros, Marcelo B.; Mérida Guzmán, María A.; Mikatadze-Pantsulaia, Tsira; Ibrahim Mohamed, El Tahir; Monteros-Altamirano, Álvaro; Morales, Aura; Müller, Jonas V.; Mulumba, John W.; Nersesyan, Anush; Nóbrega, Humberto; Nyamongo, Desterio O.; Obreza, Matija; Okere, Anthony U.; Orsenigo, Simone; Ortega-Klose, Fernando; Papikyan, Astghik; Pearce, Timothy R.; Pinheiro de Carvalho, Miguel A. A.; Prohens, Jaime; Rossi, Graziano; Salas, Alberto; Singh Shrestha, Deepa; Uddin Siddiqui, Sadar; Smith, Paul P.; Sotomayor Melo, Diego Alejandro; Tacán, Marcelo; Tapia, César; Toledo, Álvaro; Toll, Jane; Vu, Dang Toan; Tuong, Dang Vu; Way, Michael J.; Yazbek, Mariana; Zorrilla Cisneros, Cinthya; Kilian, Benjamin
The Adapting Agriculture to Climate Change Project set out to improve the diversity, quantity, and accessibility of germplasm collections of crop wild relatives (CWR). Between 2013 and 2018, partners in 25 countries, heirs to the globetrotting legacy of Nikolai Vavilov, undertook seed collecting expeditions targeting CWR of 28 crops of global significance for agriculture. Here, we describe the implementation of the 25 national collecting programs and present the key results. A total of 4587 unique seed samples from at least 355 CWR taxa were collected, conserved ex situ, safety duplicated in national and international genebanks, and made available through the Multilateral System (MLS) of the International Treaty on Plant Genetic Resources for Food and Agriculture (Plant Treaty). Collections of CWR were made for all 28 targeted crops. Potato and eggplant were the most collected genepools, although the greatest number of primary genepool collections were made for rice. Overall, alfalfa, Bambara groundnut, grass pea and wheat were the genepools for which targets were best achieved. Several of the newly collected samples have already been used in pre-breeding programs to adapt crops to future challenges.
Addressing challenges in promoting the use of animal-origin textile fibers for mitigating microplastic pollution on earth
(MedCrave Group, 2023-11-27) Frank, Eduardo Narciso; Hick, Michel Victor Hubert; Flores Gutiérrez, Alfonso; Castillo, Maria Flavia; Prieto, Alejandro; Castillo, Melina; Gomez, Maria Betina; Martinez, Adrian; Mamani Cato, Ruben Herberht; Seghetti Frondizi, Diego
The aim of this review is to address the issue of textile-origin microplastics and to provide possible solutions that can be propose through a scientific and development program. The constant expansion of textile production and consumption, driven by population growth and the ubiquity of fast fashion, has triggered environmental contamination resulting from the release of fiber fragments during the washing and use of clothing and household textiles. These fragments, ranging in size from 1 μm to 5 mm, pose a novel source of pollution that not only threatens the health of aquatic animals when ingested but also risks human food safety by infiltrating the food chain. Despite the growing awareness of the environmental impacts of these plastic microfibers (MP) from synthetic textiles, this study departs from conventional actions focused on mitigating microplastic pollution. Instead, it focuses on proposing specific measures to reduce MP emissions and addresses the even greater challenge of replacing these plastic microfibers with animal-origin textile fibers. This approach emerges as a promising and sustainable alternative to counteract the negative environmental impact of the contemporary fashion industry. The essence of this challenge lies in determining the competitiveness of animal-origin textile fibers against MP. To achieve this goal, specific actions are proposed, including the need to reduce both the environmental impact and costs associated with processing animal fibers. The study also emphasizes the importance of effectively highlighting the biological and textile advantages of these fibers to encourage their acceptance and adoption in the competitive textile market. This project addresses concrete strategies to promote the use of animal-origin textile fibers. It suggests optimizing the cleaning process and genetically improving these fibers, considering key aspects such as quality and color. The research also highlights the potential contribution to sustainability certification and well-being associated with the use of animal-origin textile fibers, providing a solid foundation for their adoption in the textile industry. In conclusion, this work not only departs from traditional measures focused on microplastics to address MP release but ventures into more ambitious territory by proposing increased use of animal-origin textile fibers as a path to a more sustainable fashion. Specific actions are outlined to enhance the competitiveness of these fibers in the current market, emphasizing their potential contribution to reducing textile pollution and thus strengthening the transition to more environmentally responsible practices in the fashion industry.
The advance of Fusarium wilt tropical race 4 in Musaceae of Latin America and the Caribbean: Current situation
(MDPI, 2023-02-08) Martínez, Gustavo; Olivares, Barlin Orlando; Rey, Juan Carlos; Rojas Llanque, Juan Carlos; Cardenas, Jaime; Muentes, Carlos; Dawson, Carolina
The fungus Fusarium oxysporum f. sp. cubense tropical race 4 (syn. Fusarium odoratissimum) (Foc TR4) causes vascular wilt in Musaceae plants and is considered the most lethal for these crops. In Latin America and the Caribbean (LAC), it was reported for the first time in Colombia (2019), later in Peru (2021), and recently declared in Venezuela (2023). This work aimed to analyze the evolution of Foc TR4 in Musaceae in LAC between 2018 and 2022. This perspective contains a selection of topics related to Foc TR4 in LAC that address and describe (i) the threat of Foc TR4 in LAC, (ii) a bibliometric analysis of the scientific production of Foc TR4 in LAC, (iii) the current situation of Foc TR4 in Colombia, Peru, and Venezuela, (iv) medium-term prospects in LAC member countries, and (v) export trade and local food security. In this study, the presence of Foc TR4 in Venezuela and the possible consequences of the production of Musaceae in the long term were reported for the first time. In conclusion, TR4 is a major threat to banana production in Latin America and the world, and it is important to take measures to control the spread of the fungus and minimize its impact on the banana industry. It is important to keep working on the control of Foc TR4, which requires the participation of the local and international industry, researchers, and consumers, among others, to prevent the disappearance of bananas.
Age-dependent changes in protist and fungal microbiota in a peruvian cattle genetic nucleus
(MDPI, 2024-08-14) Estrada Cañari, Richard; Romero Avila, Yolanda Madelein; Quilcate Pairazamán, Carlos Enrique; Dipaz Berrocal, Deisy Juana; Alejos Asencio, Carol Silvia; Leon Trinidad, Silvia Eliana; Alvarez García, Wesley Yusmein; Rojas, Diorman; Alvarado, Wigoberto; Maicelo Quintana, Jorge Luis; Arbizu, Carlos Irvin
In this research, the connection between age and microbial diversity in cattle was explored, revealing significant changes in both protist diversity and fungal microbiota composition with age. Using fecal samples from 21 Simmental cattle, microbial communities were analyzed through 18S rRNA gene sequencing. Results indicated significant differences in alpha protist diversity among the three age groups, while fungal composition varied notably with age and was linked to hematological parameters. Despite the stability of fungal alpha diversity, compositional changes suggest the gut as a stable niche for microbial colonization influenced by diet, clinical parameters, and microbial interactions. All cattle were maintained on a consistent diet, tailored to meet the specific nutritional needs of each age group. These findings emphasize the importance of understanding age-related microbial dynamics to enhance livestock management and animal health, contributing to broader ecological and biomedical research. This study was limited by the lack of comprehensive metabolic analyses correlating microbiota changes with specific age-related variations, indicating a need for further research in this área.
Agricultural reuse of the digestate from low-cost tubular digesters in rural Andean communities
(El Sevier, 2011-09-07) Garfí, Marianna; Gelman, Pau; Comas, Jordi; Carrasco Chilón, William Leoncio; Ferrer, Ivet
This research aimed at assessing the properties of guinea pig manure digestate from low-cost tubular digesters for crops fertilization in rural Andean communities. To this end, field trials were carried out to evaluate the effect of the digestate on two common Andean crops: potato (Solanum tuberosum) and forage (Lolium multiflorum and Trifolium pratense L.). The potato yield (20–25 t ha−1) increased by 27.5% with digestate, by 15.1% with pre-compost and by 10.3% with the mixture, compared to the control. The forage yield (20–21 t ha−1) increased by 1.4% with digestate – 50% dose, and by 8.8% with digestate – 100% dose and digestate – 150% dose, compared to the control. The results suggest that the digestate is an appropriate substitute of manure pre-compost for potato fertilization. The results with forage indicate that it can be applied in a range of doses, according to the amount produced by the digester. Currently, manure is either used for cooking or as fertilizer. With low-cost tubular digesters implementation, it could be used to feed the digester, using the digestate for crops fertilization and biogas for cooking; improving household living conditions and protecting the environment. Since soil properties in rural Andean communities differ from experimental layouts, the effect of fertilizers should be re-evaluated in-situ in future research studies.
Agro-morphological characteristics and sensory evaluation of native peruvian chili peppers
(David Publishing Company, 2016-09-28) Patel, Kirti; Calderon, Rosa; Asencios, Edgard; Vilchez Palomino, Dioliza; Marcelo Salvador, Mavel Nansi; Rojas, Rosario
In this work, 31 native Peruvian chili peppers were evaluated for their agro-morphological characteristics and sensory attributes. The descriptive sensory analysis (DSA) was used to evaluate the 31 chili pepper samples based on their acid, bell pepper, sweet, tomato, apple, citrus, fruity, herbal, oregano and passion fruit attributes. The data obtained from the DSA enabled the grouping of these 31 chili peppers into six groups based on their different sensory attributes, such as fruity, bell pepper and herbal. The most abundant group (12 of the 31) had the bell pepper, apple, herbal, fruity and sweet attributes. Each group contained different species and different physical appearances, indicating that species or different forms of chili pepper do not define that sensory attribute. Considering the fact that Peruvian peppers are in high demand, the results achieved would be useful for growers, producers and chefs, as well as for further breeding activities.
Agro-morphological characterization and diversity analysis of Coffea arabica germplasm collection from INIA, Peru
(John Wiley & Sons Inc., 2023-06-06) Paredes Espinosa, Richard; Gutiérrez Reynoso, Dina Lida; Atoche Garay, Diego Fernando; Mansilla Córdova, Pedro Javier ; Abad Romaní, Yudi Gertrudis; Girón Aguilar, Rita Carolina; Flores Torres, Itala; Montañez Artica, Ana Gabriela; Arbizu Berrocal, Carlos Irvin; Amasifuen Guerra, Carlos Alberto; Maicelo Quintana, Jorge Luis; Poemape Tuesta, Carlos Augusto; Guerrero Abad, Juan Carlos
Coffee (Coffea arabica L.) plays a major role in the economy of Peru and the world. The present study aims to elucidate the agro-morphological variability of coffee genotypes maintained in the INIA´s germplasm collection. Therefore, 20 vegetative, reproductive, and phytosanitary traits of 162 coffee accessions of INIA’s germplasm collection were evaluated and analyzed. Correlation results indicate that a simultaneous selection of characters, such as number of branches per plant, number of nodes per branch, leaf area and weight of a hundred fruits, can contribute to increase coffee yields. Additionally, coffee yield was negatively correlated with the incidence and severity of coffee leaf rust, and interestingly the occurrence of small and compact coffee plants with high resistance to the disease was also found. The analysis of Tocher and Mahalanobis D2 determined the formation of 10 groups of divergent coffee accessions; where clusters 1 (accession codes 20, 29, 38, 54, 67, 71, 117, 24, 26 and 27), 5 (accession codes 46 and 53), 9 (accession code 159), and 10 (accession code 203) group promising accessions that can be used in breeding programs. Principal component analysis (PCA) showed that at least five of its principal components managed to explain 70.01% of the total variation in the collection. Finally, the high coefficients obtained for the phenotypic, genotypic and heritability variation confirm the existence of additive genes in the evaluated population, that would ensure the success of coffee breeding programs based on the selection of traits of agronomic importance.
Agrobiodiversidad, género y cambio climático en la cuenca del río Mantaro
(Soluciones Prácticas-ITDG, 2009-07-31) Cuellar Bautista, José Eloy; Medina Hinostroza, Tulio Cecilio
[ES] En la cuenca del río Mantaro se pueden percibir las iniciales consecuencias del cambio climático sobre la agricultura y otras actividades socioeconómicas. La vulnerabilidad de la cuenca se verá agravada por la excesiva parcelación de la tierra de uso agrícola y eventos climáticos extremos cuya tendencia aumenta progresivamente. El presente artículo presenta un profundo análisis socioeconómico y climático de la problemática del valle y establece los pasos necesarios para construir un proceso de mitigación y adaptación al cambio climático.--- [EN] In the Mantaro river basin, there is evidence of the initial consequences of climate change on agricultural and other socio-economic activities. The vulnerability of the river basin will be further aggravated by the excessive subdivision of farmland and extreme climatic events, the tendencies of which are gradually increasing. This article contains a thorough socio-economic and climatic analysis of the problems in the valley and establishes the steps required to build a climate change mitigation and adaptation process.
Agromorphological characterization of cacao (Theobroma cacao L.) accessions from the germplasm bank of the National Institute of Agrarian Innovation, Peru
(Elsevier, 2022-10-04) Vásquez García, Jheiner; Santos Peláez, Julio César; Malqui Ramos, Roiber; Vigo Mestanza, Carmen Natividad; Alvarado Chuqui, Wigoberto; Bobadilla Rivera, Leidy Gheraldine
Agromorphological characterization of cacao accessions in Peru is currently an important tool in the conservation and genetic improvement of cacao germplasm. The objective of this study was to carry out the morphological and agronomic characterization of 113 cacao accessions from the Huarangopampa germplasm bank. Tree, leaf, flower, fruit and seed descriptors were used. The data collected were processed by descriptive statistics using multivariate techniques. Five groups were formed according to similar characteristics. The accessions of group 1 are vigorous trees with an pod index of 19.27 pods/kg of seeds; the groups that presented better differential characteristics were group 2 with erect tree architecture, intermediate vigorousness, purple seed color and pod index of 20.07 pods/kg of seeds and group 3, which had the highest number of accessions with the lowest pod index of 18.77 pods/kg of seeds, besides being vigorous trees and having purple seeds. On the other hand, group 4 presented a particular characteristic of white seed color and high pod indexes with 22.11 pods/kg of seeds. Finally, group 5 accessions were characterized by intermediate tree architecture and vigor with an pod index of 21.3 pods/kg of seeds. The morphoagronomic characterization constitutes a first advance in the identification of cacaos with potential for genetic improvement and advances in the Peruvian chocolate industry.
Agronomic behavior of cowpea varieties in non-alluvial soils of the Peruvian Amazon
(Universidade Federal Rural da Amazônia, 2022-05-06) Angulo Villacorta, Carlos Darwin; Mathios Flores, Marco Antonio; Sangama Arirama, Misael Nemecio; Racchumi García, Alfredo
Grain legumes in tropical areas are of great importance as a food source, especially in rural communities. Little is known about their adaptability and behavior in non-alluvial soils of the Peruvian Amazon. Our objective was to assess the agronomic behavior of cowpea varieties in non-alluvial soils. The experiment was performed in completely randomized design with four treatments and four repetitions. Each experimental unit consisted of five rows of 6m length by 0.5m between plants and 1m between rows. The variables evaluated were: plant height (cm), number of pods, pod length (cm), number of graind per pod, grain length (mm), grain width (mm), and weight of 100 seeds (g). An analysis of variance and Tukey’ test at 5% probability were used to determine significant differences between treatments. Results showed significant differences between varieties according to the evaluated variables. The red cowpea presented significant differrences for the agronomic characteristics in plant height (62.83cm), grain length (8.68mm), and weight of 100 seeds (16.87g) in relation to the other varieties. The Chiclayo Olho Preto variety stood out only in grain width (5.46mm), and the Chiclayo Vermelho in pod length (19.58cm). The red cowpea has the potential to be cultivated preliminarily in larger-scale areas in the Yurimaguas district
Agronomic behaviour and chemical composition of three varieties of Pennisetum sp in the Peruvian Tropics
(Hindawi, 2023-10-23) Watanabe Escobedo, Enrique Susumu; Juancho Ramirez, Ronald; Alvarado Atanacio, Carlos Enrique; López Rengifo, Debora; Injante Silva, Pedro Hugo; Ampuero Trigoso, Gustavo; Saucedo Uriarte, José Américo
Livestock production in the Peruvian tropics is mainly based on grazing and cutting grasses, which have variable productive and nutritional characteristics according to the variety of grasses, agronomic management, and climatic conditions. The objective of this research was to compare the agronomic behaviour and chemical composition of three varieties of Pennisetum sp (Pennisetum purpureum Schum cv. Cameroon or Cameroon grass, Pennisetum sp. or Maralfalfa, and Pennisetum purpureum × Pennisetum typhoides or King grass). The experiment was carried out at the Estación Experimental Agraria “Campo Verde,” Ucayali, Peru, under a randomized complete block design (RCBD) with a 3 (Pennisetum sp. variety) × 3 (cutting age) factorial arrangement and 4 replicates each. Thirty-six subplots were installed, and treatments were randomly assigned, using vegetative seed with three buds each. Measurements of agronomic behaviour and chemical analysis were carried out in the 8th, 12th, and 16th week. The data were subjected to ANOVA and multiple comparisons with the Duncan and Kruskal–Wallis tests ( < 0.05) and correlations using Pearson coefficients ( < 0.05). The agronomic behaviour did not vary between Pennisetum sp. varieties, but according to cutting age, Cameroon grass produced greater plant height, biomass, dry matter, and Mg content at the 8th week. The highest concentrations of N (2.38 ± 0.11%), P (0.18 ± 0.01%), and Ca (0.70 ± 0.03%) were recorded at the 8th week, Zn (27.33 ± 1.91 mg/kg) at the 12th week, and Mg (0.26 ± 0.02%) at the 16th week. A negative correlation was found between biomass and the stem-leaf ratio at the 12th week because the first leaves of plants died, but the stems increased in size. It is concluded that, at an early cutting age in varieties of Pennisetum sp., lower biomass and dry matter result, but higher content of N, P, Ca, and Fe; and at a late cutting age, it shows greater biomass, dry matter, Cu, and Mg content.
Aislamiento de ADN genómico de Myrciaria dubia (HBK) "camu camu" apropiado para análisis moleculares
(Universidad Científica del Perú, 2012-06-29) Castro Gómez, Juan Carlos; Cobos Ruiz, Marianela; Ramírez Saavedra, Roberson; Imán Correa, Sixto Alfredo
Myrciaria dubia “camu-camu”, una especie nativa de la Amazonía que produce frutos con alto contenido de vitamina C y otras sustancias importantes. Sin embargo, los estudios moleculares de esta planta son escasos, por falta de un protocolo reproducible para purificar sus ácidos nucléicos. El objetivo de este trabajo fue establecer un protocolo para aislar el ADN genómico a partir de hojas de M. dubia, apropiado para análisis moleculares. El ADN se purificó con un protocolo modificado, la calidad y cantidad se estimó por espectrofotometría y electroforesis en gel de agarosa. Adicionalmente, la calidad se evaluó mediante RAPD. El ratio de calidad (A260/A280) promedio del ADN fue 1.9±0.1 y el espectro de absorción UV/Vis presentó un único pico de máxima absorbancia a 260nm. Mediante electroforesis el ADN fue íntegro y sin ARN. También, la síntesis de amplicones RAPD nos sugiere ausencia de inhibidores para polimerasas. La concentración promedio del ADN fue 99±33 ng/ml y el rendimiento promedio fue 237±80 mg ADN/g hoja. En conclusión, se ha establecido un protocolo de aislamiento de ADN genómico a partir de hojas de Myrciaria dubia “camu camu”, caracterizado por permitirnos obtener ADN de alta calidad y cantidad suficiente para análisis moleculares como el RAPD.
Aislamiento, identificación y evaluación in vitro de aislados nativos de bacillus, trichoderma y streptomyces con potencial para el biocontrol de los hongos de la madera de la vid
(Universidad Autónoma de Yucatán, 2022-05-11) Morales Pizarro, Arturo; Javier Alva, Javier; Álvarez, L. A.; Mayta Obos, Rocío; Aguilar Anccota, René; Peña Castillo, Ricardo; Chanduvi García, Roger; Lindo Seminario, David Enrique
Antecedentes: El biocontrol de las enfermedades fúngicas de la madera de la vid (EMV), es una alternativa amigable frente al control químico causante de resistencia y/o contaminación del ecosistema. Objetivo: aislar microorganismos nativos de raíz y rizósfera de vid con potencial de biocontrol de hongos de la madera de la vid. Metodología: Se estudió la “competencia por sustrato” (CS) con la escala de Bell, “el tiempo de contacto” (TC) entre antagonista-patógeno” (días) y la antibiosis por el porcentaje de inhibición del crecimiento (PIC). Resultados: Se aislaron e identificaron: siete-cepas de Trichoderma spp. (raíz: Tr-1, Tr-2, Tr3, Tr-6; y rizósfera: Tr-4, Tr-5, Tr-7); seis-Bacillus spp. (raíz: Bac-4, Bac-5, Bac-6; y rizósfera: Bac-1, Bac-2, Bac-3); y dos-Streptomyces sp. (rizósfera: Act-1, Act-2). Los resultados de la CS evaluados con la escala de Bell demostraron a Tr-1, Tr-7, T.atv (Trichoderma atroviride) y T-22 (Trichoderma harzianum) se ubicaron en la clase 1 frente a Lasiodiplodia theobromae y, todos los aislados se ubicaron en la clase 1 frente a Campylocarpon pseudofasciculare y Phaeoacremonium parasiticum. El TC de Trichoderma fue 2 días (L. theobromae) y 3 días (C. pseudofasciculare y P. parasiticum). En la antibiosis Tr-5 (Trichoderma) y Bac-3 (Bacillus) tuvieron el mayor PIC (>50%) frente a los patógenos. El aislado Act-2 (Streptomyces sp.) presentó PIC, >70% (L. theobromae), >40% (C. pseudofasciculare) y >30% (P. parasiticum). Implicaciones: El uso del control biológico en el manejo de enfermedades de la madera de la vid es una herramienta eficaz, y puede integrarse en una estrategia de manejo integrado de estas patologías. Conclusiones: Los aislados nativos Bac-3 (Bacillus spp.), Tr-5 (Trichoderma spp.), Act-2 (Streptomyces sp.) demostraron control in vitro frente a los patógenos L. theobromae, C. pseudofasciculare y P. parasiticum, patógenos asociados a enfermedades de la madera de la vid.

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